Coding

Part:BBa_K2560259

Designed by: Daniel Marchal   Group: iGEM18_Marburg   (2018-09-30)
Revision as of 15:13, 30 September 2018 by Marchal (Talk | contribs)


mcr gene for Malonyl-CoA Reductase from Sulfolobus tokodaii

mcr encodes the enzyme Malonyl-CoA Reductase from Sulfolobus tokodaii. This enzyme is involved in the 3-hydroxypropionate cycle for CO2 fixation and converts malonyl-CoA into 3-oxopropanoic acid (Strauss & Fuchs, 1993). In a two-step reaction malonyl-CoA is first reduced to 3-oxopropanoic acid and then further reduced to 3-hydroxypropionic acid.

BBa K2560260 Mcr pathway.png

For this part, mcr was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 626
    Illegal BglII site found at 819
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None