Part:BBa_K2560260

mcr gene for Malonyl-CoA Reductase from Chloroflexus aurantiacus

mcr encoded the enzyme Malony-CoA Reductase from C. aurantiacus. This enzyme is involved in the 3-hydroxypropionate cycle for CO2 fixation and converts malonyl-CoA into 3-hydroxypropionic acid (Strauss & Fuchs, 1993). In a two-step reaction malonyl-CoA is first reduced to 3-oxopropanoic acid and then further reduced to 3-hydroxypropionic acid.

For this part, mcr was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI.

It was shown that a splitted version of Mcr with separated C- and N-terminal domains increases enzyme activity (Liu et al., 2013). If these splitted genes shall be used take BBa_K2560261 and BBa_K2560262.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1932
  Illegal NheI site found at 3334
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 885
  Illegal BamHI site found at 213
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1188
  Illegal NgoMIV site found at 2005
  Illegal NgoMIV site found at 3619
  Illegal AgeI site found at 1270
  Illegal AgeI site found at 3168
  
• 1000
  COMPATIBLE WITH RFC[1000]