Coding
Part:BBa_K2686001:Experience
Designed by: Thomas Jordan Group: iGEM18_EPFL (2018-09-30)
Revision as of 12:25, 16 October 2018 by Caffeine4Life (Talk | contribs)
The part was designed and constructed in a pET14 vector. The sequence was confirmed using sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32.96kDa for the monomer and 1.98MDa for the 60-mer). DLS measurements also show that our protein cage self-assembles into the 60-mer.
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UNIQ41571515454f968c-partinfo-00000000-QINU UNIQ41571515454f968c-partinfo-00000001-QINU