Coding

Part:BBa_K2570001:Design

Designed by: Luying He   Group: iGEM18_FJNU-China   (2018-09-13)
Revision as of 07:20, 30 September 2018 by Who care (Talk | contribs) (→‎References)


Aro10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ordered the part DNA from a synthesis company and had codon optimization of the sequence. Mutation of restriction sites EcoRⅠ, SpeⅠand PstⅠ.

Source

The phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3).

https://www.ncbi.nlm.nih.gov/protein/NP_010668.3?report=genbank&log$=protalign&blast_rank=1&RID=V0V04K3B015

References

Wang, P., Yang, X., Lin, B., Huang, J., & Tao, Y. (2017). Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol. Metabolic Engineering, 44(August), 143–149.