Project

Part:BBa_K2555003

Designed by: Tingyue Li   Group: iGEM18_BFSUICC-China   (2018-09-20)
Revision as of 07:01, 29 September 2018 by Duhongmei (Talk | contribs)


pBAD-RBS-sfGFP

BBa_K2555003 contains PBAD promoter, RBS, and superfolder GFP and Terminator. This part is used to indirectly reflect the amount of CueR protein in Part BBa_K2555004 expression in our project.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1085
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1067
    Illegal SapI.rc site found at 1355


[edit]
Categories
Parameters
None