DNA

Part:BBa_K2856001:Design

Designed by: Zhuwei Shi   Group: iGEM18_H14Z1_Hangzhou   (2018-09-29)
Revision as of 07:12, 29 September 2018 by Zy007 (Talk | contribs) (→‎Design Notes)


Bifunctional glutamate-cysteine ligase/glutathione synthase (gshF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

there are EcoRI，XbaI,SpeI restriction sites in original coding squence.So we ask the company to de novo synthsis this squence to remove the illegal resitriction sites

Source

this gene is amplified from Streptococcus thermophilus genome

References