Coding

Part:BBa_K2656013

Designed by: Adrián Requena Gutiérrez, Carolina Ropero   Group: iGEM18_Valencia_UPV   (2018-09-20)
Revision as of 19:09, 28 September 2018 by Adrireq (Talk | contribs)


sfGFP Coding Sequence

Part BBa_I746916 standarized as Phytobrick. Super Folder Green Fluorescent Protein. It can be used to assemble transcriptional units with the Golden Gate assembly method, and also, it can be used as a Biobrick.

We have obtained the emission and excitation spectra of this protein following this protocol (REF) and with the parameters of the table 1 we have obtained the results of the graphic 1:

Parameter Value
Sample number 0
Minimum excitation wavelenght (nm) 0
Maximum excitation wavelenght (nm) 0
Emission wavelenght (nm) 0
Minimum emission wavelenght (nm) 0
Maximum emission wavelenght (nm) 0
Excitation wavelenght (nm) 0
Gain (G) 0

Table 1.

Frame


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 14


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter
//function/reporter/fluorescence
//test
Parameters
None