Coding

Part:BBa_K2560253:Design

Designed by: Daniel Marchal   Group: iGEM18_Marburg   (2018-09-28)
Revision as of 19:11, 28 September 2018 by Marchal (Talk | contribs)


birA from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

Source of the part:

Genome: Corynebacterium glutamicum ATCC 13032

Accession number of gene: NCgl0679

Accession number of encoded protein: NP_599941.1

birA was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI


References

Samols. D.. Thornton, C.H., Murtif. V.L.. Kumar, G.K.. Haase. F,C. and Wood. H.G. (1988) J. Biol. Chem. 263. 6461-6464.