Coding

Part:BBa_K2560255:Design

Designed by: Daniel Marchal   Group: iGEM18_Marburg   (2018-09-28)
Revision as of 19:27, 28 September 2018 by Marchal (Talk | contribs) (→‎Design Notes)


accD subunit from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the accD gene of C. glutamicum (NCgl0678) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011). If accD shall be used with an 5' tag use this part, if there is no need for tagging, use BBa_K2560252.

GGTCTCGGATG-coding_region-GCTTTGAGACC

The sequence was codonoptimized for V. natriegens ATCC 14048.

Source

Source of the part:

Genome: Corynebacterium glutamicum ATCC 13032

Accession number of gene: NCgl0678

Accession number of encoded protein: NP_599940.1

accD was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002via BsmBI

References

Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765