Tag
map20 sign

Part:BBa_K2871000:Design

Designed by: Nuttawat Leelahakorn   Group: iGEM18_UCopenhagen   (2018-09-24)
Revision as of 20:22, 24 September 2018 by Attilauslu (Talk | contribs) (→‎References)


Map20, T3SS export signal peptide from Map gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The paper described by Charpentier & Oswald (2004), showed that a 20 amino acid sequence was sufficient albeit not the best. The short length however seemed attractive to us, because we wanted to minimize folding interference between the signal sequence and the intended protein fused to the signal sequence.


Source

Synthetic sequence deduced from Amino acid Sequence from the Enteropathogenic E. coli strain E22. Described in the paper Charpentier & Oswald, 2004. 'Identification of the secretion and translocation domain of the enteropathogenic end enterohemorrhagic escherichia coli effector cif, using TEM-1-beta-lactamase as a new fluorescence-Based Reporter'

References

DOI: 10.1128/JB.186.16.5486-5495.2004 https://jb.asm.org/content/186/16/5486