Regulatory

Part:BBa_K2688025

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-09-23)
Revision as of 19:00, 12 October 2018 by Briand (Talk | contribs)

J23108_corrected

J23108 promoter is part of the Chris Anderson’s collection of plasmid. The pSB1C3-BBa_J23108 was obtained by PCR mutagenesis of the pSB1C3-BBa_J23119.

This biobrick is a corrected version of the initial biobrick (BBa_J23108) obtained from (2018 – Plate 4 – 4C) position which contains extra-sequences (notably SpeI forbidden sites).

This corrected version is devoid of extra-sequences and is therefore convenient for a direct biobrick cloning of RBS-CDS under the control of J23108 promoter.

For details on the process, see http://2018.igem.org/Team:GO_Paris-Saclay/Improve


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None