Coding

Part:BBa_K2598000:Design

Designed by: Xu Zepeng   Group: iGEM18_UCAS-China   (2018-09-18)
Revision as of 15:24, 25 September 2018 by Gaoyuanli1998 (Talk | contribs) (→‎References)


T7 core fragment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1819
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We exploited this part along with sigma fragments to achieve accurate control over multiple outputs.


Source

This part is designed by Segallshapiro, T. H., et al based on T7 polymerase

References

Segall-Shapiro, T. H., Meyer, A. J., Ellington, A. D., Sontag, E. D. & Voigt, C. A. A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol 10, 742, (2014).