Coding

Part:BBa_K2586020

Designed by: Janek Meißner   Group: iGEM18_Goettingen   (2018-09-13)
Revision as of 10:44, 13 September 2018 by Dschannek96 (Talk | contribs)


AroA*: Resistant aroA-variant in Escherichia coli

This part encodes a resistant variant to the native aroA gene in Escherichia coli. The resistance occurs due to 2 point mutations within the DNA sequence. At position 287 a Guanin was changed to a Cytosin and at position 547 a Guanin was changed to an Adenin.

This part can be used to confer glyphosate resistance to bacteria, as the mutation decreases the interaction of the herbicide with the EPSP-Synthase, which is encoded by the native aroA gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 346
    Illegal AgeI site found at 787
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1021


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Parameters
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