Translational_Unit
Csy4-Q104A
Part:BBa_K2615004:Design
Designed by: Yunqian Zhang Group: iGEM18_OUC-China (2018-09-01)
Csy4-Q104A, the No.2 member of Csy4 family.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 377
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 93
Design Notes
We designed this part by point mutation. We changed the CAG(encoding Gln) to GCG(encoding Ala) on the 104th site based on wild type Csy4.
Source
The cleavage of Cys4 releases a cis-repressive RNA module (crRNA,paired with RBS) from the masked ribosome binding site (RBS), which subsequently allows the downstream translation initiation. A Ribosome Binding Site (RBS) is an RNA sequence to which ribosomes can bind and initiate translation. Combined RBS and Cys4, it can be more convenient for other iGEMers to use this composite part without putting a RBS aequence on the upstream of Cys4 coding sequence.