Coding

Part:BBa_K2586001

Designed by: Robert Warneke, Janek Meißner   Group: iGEM18_Goettingen   (2018-09-10)
Revision as of 06:42, 11 September 2018 by FMCommmichau (Talk | contribs)


GltT: glutamate and glyphosate transporter in B. subtilis

This part is coding for a glutamate and glyphosate transporter. The DNA sequence of the gltT gene is coding for the GltT transporter, which is responsible for glutamate uptake into the cell. We found that GltT is also involved in glyphosate uptake in the Gram-positive model bacterium Bacillus subtilis.

In the abscence of GltT causes the bacteria to develop a resistance against glyphosate, as less of this herbicide and antibiotic are transported into the cell. This means that this part has the potential to be involved in the development of glyphosate resistant detection and inactivation systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1123
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part/nominee
//chassis/prokaryote/bsubtilis
Parameters
resistance