Coding

Part:BBa_K2586001:Design

Designed by: Robert Warneke, Janek Meiner   Group: iGEM18_Goettingen   (2018-09-10)
Revision as of 10:12, 10 September 2018 by Dschannek96 (Talk | contribs) (→‎Design Notes)


GltT: glutamate and glyphosate transporter in B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.

Source

This part is a PCR product made through de novo synthesis in the lab.

References