Composite
ZntA

Part:BBa_K2832008

Designed by: Michael Taylor and Arianna Sigalos   Group: iGEM18_FSU   (2018-09-09)


ZntR Inducible mRFP1 Generator ( ZntR ---> ZntAp ---> mRFP1 )

Mechanosensitive channels open to mechanical force. Sound being the mechanical force can open the channel. Zinc ions, among others, enters the cell. Zinc cations bind to zntR, zinc-dependent transcription factor. Active zntR binds to zntAp. zntAp upregulates mRFP to show correct induction. Natural systems, zntA upregulates zntA, a zinc export system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
    Illegal AgeI site found at 765
    Illegal AgeI site found at 877
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None