Part:BBa_K1062004:Experience

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••• USTC_China iGEM13

In USTC_China iGEM13's project,we bulit the N-terminal TD1 modified GFP to make it is capable of transporting through skin barrier, then induced expression in BL21 and Bacillus Subtilis WB800N with IPTG and tested positive by SDS-PAGE and ultraviolet ultraviolet spectrometry. Students in Wen lab validated the efficient transdermal function of N-terminal TD1 modified GFP(not BBa_E0040 ). figure 1 SDS-PAGE of N-terminal TD1 modified GFP figure 2 Fluorescence detection of N-terminal TD1 modified GFP expression with and without IPTG induction figure 3 Our Transdermal test device figure 4 Results of transdermal test of N-terminal TD1 modified GFP and unmodified GFP