Project

Part:BBa_I759027

Designed by: Josh Michener   Group: iGEM07_Caltech   (2007-10-24)
Revision as of 04:30, 27 October 2007 by Cal (Talk | contribs)

cis3-repressed, tet-regulated Q

This part has the Q construct from I759003 with the cis repressor cis3 cloned in to replace the spacer. The transcript will form a cis-hairpin loop and prevent translation in the absence of a riboregulator trans sequence, which binds to and opens up the cis-hairpin, allowing the ribosome to access the RBS site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 890
    Illegal NheI site found at 913
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None