Reporter
mRFP1

Part:BBa_K2835001

Designed by: Hanna Norbäck, Emma Larsson   Group: iGEM18_Stockholm   (2018-08-21)
Revision as of 07:29, 28 September 2018 by Hnorback (Talk | contribs)


Constitutive reporter: strong promoter + RBS + mutant of mRFP1 (RFC25 compatible)

The protein coding sequence for iGEM11_Uppsala's highly engineered mutant RFP with strong RBS made compatible with the iGEM RFC 25 assembly standard. and thereby all iGEM assembly standards. This improvement was made by iGEM18_Stockholm .


Rfpminiprep.jpeg BothRFP.jpeg

The colonies will turn red after 18 hours.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/rfp
//chassis/prokaryote/ecoli
//function/reporter
//function/reporter/color
//function/reporter/fluorescence
Parameters
colorRed
emission607 nm
excitation584 nm