Composite

Part:BBa_K2624005

Designed by: Shuyao Zhou, Huandi Xu, Junqing Zhang, Yali Wu   Group: iGEM18_CPU_CHINA   (2018-08-17)
Revision as of 12:32, 18 August 2018 by KKehuandiCPU (Talk | contribs)


hulc-pri-miRNA(MAP4K4)

This composite consists of two parts. One is the core promoter of the gene highly up-regulated in liver cancer (HULC), the other designed to encode a primary microRNA analogue targeting MAP4K4. This expression unit is up-regulated in hepatocellular carcinoma and liver cancer cell lines at transcription level, hence constitutive knock-down of MAP4K4 if such cells are transfected with.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 130


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Categories
Parameters
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