Coding

Part:BBa_K2719008:Design

Designed by: Andrea Pamela Jimenez Tapia & Rodrigo Valencia Ocampo   Group: iGEM18_TecCEM   (2018-08-08)
Revision as of 12:44, 17 October 2018 by JRUEDA (Talk | contribs)


Leptin

This is originated from Hommo sapiens, the original sequence is from this species but was optimized for E.coli BL21 (DE3) and to be cloned by RFC10 on pSB1C3. It mainly consists on a-helix. An histidine tag (6xHis) was added to the protein to facilitate its purification.

"T--TecCEM--Lep3DStructure.png"

Figure 7. Leptin 3D prediction modelled with Swiss Model


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for E.coli.


Source

NCBI Genomic Library

References