Device

Part:BBa_K2719005:Design

Designed by: Karla Soto Blas   Group: iGEM18_TecCEM   (2018-08-08)
Revision as of 12:47, 17 October 2018 by AKARAM (Talk | contribs) (→‎Design Notes)


Tenascin Domain V Expression Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 416


Design Notes

For the design if this part the following subparts: an IPTG inducible T7 promoter (BBa_R0184), in order to maximize the recombinant protein production. An Anderson RBS (BBa_J61100), the Tenasin 5 Domain V+GST fusion protein coding site and a double terminator. This part is designed for the IPTG regulated expression of the protein in E.coli BL21 (DE3), since this strain contains an IPTG inducible RNA polymerase T7.

"T--TecCEM--BBa_K2719005Diagram.png"

Figure 1. Diagram of BBa_K2719005, tenascin 5 domain V expression device.

Source

Synthetic Part

References