Composite

Part:BBa_J100416

Designed by: Caroline Willis, Tatianna Travieso   Group: Campbell M Lab   (2018-06-21)
Revision as of 14:10, 26 June 2018 by Tatravieso (Talk | contribs)

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T7 Consensus Promoter in pClone Red

T7 consensus sequence (R0085) inserted into pClone Red (J119137), encoding for RFP. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1437
    Illegal AgeI site found at 1549
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None