Coding

Part:BBa_K2876001:Design

Designed by: Isaac Justice   Group: iGEM18_Stanford   (2018-06-22)
Revision as of 06:32, 17 October 2018 by Ijustice (Talk | contribs) (→‎Design Notes)

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Lambda CL DNA inhibitor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon optimized for E. coli, with the additional constraint of codon optimization to remove restriction sites allowing for pSB1C3 compatibility.

Source

Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0

References

Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0