Regulatory

Part:BBa_J100406

Designed by: Anthony Eckdahl, Kyla Roland   Group: Campbell M Lab   (2018-06-18)
Revision as of 20:53, 22 June 2018 by Aneckdahl (Talk | contribs)


Strong T7 Promoter (R0085) Followed by Strong C-Dog (J119028)

This is a combination of the Strong T7 Promoter <https://parts.igem.org/Part:BBa_R0085 BBa_R0085> and the Strong C-Dog Ribosome Binding Site BBa_J119028. We designed this part in order to test our mechanism for in vitro artificial selection using the SmaI enzyme. We changed the first G to a C in the C-Dog to remove the ApaI site there. We also removed the final ATG from the end of the C-dog because we already had one at the start of the SmaI gene we are using.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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