Coding

Part:BBa_M50436:Experience

Designed by: Eva Frankel   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 01:01, 12 June 2018 by Lilzhu8 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M50436

This protein can be used to speed up enterobactin synthesis slightly in E. coli. It works in tandem with entC (BBa_M50435), facilitating the transformation of chorismate to isochorismate.

User Reviews

UNIQ40d9c55a3da91d31-partinfo-00000000-QINU UNIQ40d9c55a3da91d31-partinfo-00000001-QINU

We used this sequence as a part of a 2,3-DHB Biosynthesis construct (Part:BBa_M50437). We ordered the plasmid from DNA2.0 and transformed it into E. coli, then plated the E. coli on LB + kanamycin. We saw colonies successfully grow up. We also ran a Western Blot for this protein and a saw a band of the expected size, indicating this protein was successfully synthesized. See https://parts.igem.org/Part:BBa_M50437:Experience for a detailed assessment.