Composite
pHlash

Part:BBa_M50440:Experience

Designed by: Adam C Elliott   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 17:40, 11 June 2018 by Aelliott (Talk | contribs) (→‎Applications of BBa_M50440)


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Applications of BBa_M50440

This construct can successfully be used to measure intracellular pH given by a BRET ratio in S. cerevisiae. Due to the well-characterized pH-sensitivity of Rluc8 (luciferase), we are confident that BRET readout from cpVenus (fluorophore) is indicative of intracellular pH because it was excited through Rluc8 activity.

User Reviews

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