Project
HSL Receiv
Part:BBa_I741001:Experience
Designed by: Jon Badalamenti, Lane Weaver, Tony Tascone, Lucien Weiss Group: iGEM07_Penn_State (2007-05-26)
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Applications of BBa_I741001
The HSL Receiver construct restores motility in motB knockout strain RP3087 on induction with HSL. The plasmid is sensitive to HSL concentrations as low as 1nM (publication pending). Assays have been completed by mixing cells producing AHL with "Receiver Cells" and plating on low concentrations of agar (~.3%). Confirmation of AHL production can be achieved by visualization of swarming (shown below). File:Image:Swarming cells induced with AHL.JPG
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UNIQ0d04b6965ceafd8d-partinfo-00000000-QINU UNIQ0d04b6965ceafd8d-partinfo-00000001-QINU