Coding

Part:BBa_K2442398

Designed by: Ruiyang He, James Provan   Group: iGEM17_Glasgow   (2017-09-07)
Revision as of 03:42, 2 November 2017 by Jmsprovan (Talk | contribs)

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T7 RNA Polymerase split N-term


This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.

A schematic representation of a final split-T7 RNAP AND-gate


This T7 RNA Polymerase split N-term coding sequence part did not exist, as it was directly PCR amplified with the B0032 ribosome binding site - this composite is K2442401.


Usage and Biology

This nonexistent part was used for assembly of larger composite split-T7 RNAP AND-gate constructs.

  • K2442401 B0032 + split-T7 RNAP N-term
  • K2442407 [split T7 RNAP module 1] tetR repressible promoter + T7 rnap N-term + RBS + double terminator
  • K2442410 Full split-T7 RNAP AND-gate circuit [Split RNAP module 1 + Split RNAP module 2]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None