Coding

Part:BBa_K912001

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-07-09)
Revision as of 03:06, 2 November 2017 by Ykishimotoy (Talk | contribs)


E. coli phytase (appa phytase)

Part to produce the appa e. coli phytase. This phytase acts to break down phytic acid into inositol, releasing chelated iron and other micronutrients.


HokkaidoU_Japan 2017 analyzed this part. This protein's activity falls as below graph.

Protocol: 50μl 7.5 mM Phytic acid 190μl CH3COOH Buffer

Digest phytic acid in above situation with sample phytase solution 10μl.

After digestion, using AMM solution (2ml of 10mM NH4Mo7O2.4H2O: 5N H2SO4: Acetone= 1:1:2) analyze Optical Absorbance. The more Pi are there, the more yellow AMM becomes.

T--HokkaidoU_Japan--K912001gurafu.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 688
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1213


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Parameters
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