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Part:BBa_K2364027

Designed by: Asterios Arampatzis   Group: iGEM17_Greece   (2017-10-26)
Revision as of 02:55, 2 November 2017 by Astarampatzis (Talk | contribs)


pCMV-DsRed-T145-T143-TFF4-SV40 polyA

DsRed expressed under the constitutive promoter CMV. Binding sites for the miR-145-5p, miR-143-3p and the synthetic miRNA FF4 are fused in the 3'-untranslated region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

[edit]
Categories
Parameters
None