Project

Part:BBa_K2475001

Designed by: Shawn Cody Receno   Group: iGEM17_Waterloo   (2017-10-25)
Revision as of 01:10, 2 November 2017 by LeahF (Talk | contribs)


Sup 35 Prion Domain fused to Cyan Fluorescent Protein (PrD-CFP)

This composite part consists of the Sup35 aggregation domain fused with CFP (cyan fluorescent protein), which is a fluorescent tag with a theoretical excitation peak at 430 nm and emission peak at 485 nm. This was used, in conjunction with PrD-YFP, in Förster resonance energy transfer (FRET) experiments to test if/how the aggregation domain affects the energy transfer from CFP to YFP in PSI+ (aggregate state) and psi- (soluble state).

Sup35 aggregation domain is from BBa_K2475000

Used with BBa_K2475002 to collect FRET data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None