Part:BBa_K2365515
Mutant of TEF1
Mutant of TEF1 Yeast promoter; Constitutive expression
Transcription elongation factor gene promoter of Saccharomyces cerevisiae constitutive expression promoter in the commonly used system. According to the element and registry and database of synthetic biology database bioparts for synthetic biology (http://npbiosys.scbit.org/regulatoryelementdetails/00007) design and synthesis of tef1 mutations.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 166
Fluorescence intensity was measured by flow cytometry
Measurements of specific fluorescence were performed using cells harvested from the logarithmic phase during growth in shake flasks.
Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.
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