Protein_Domain

Part:BBa_K2355032

Designed by: Chrysillis Polhaus   Group: iGEM17_DTU-Denmark   (2017-10-26)
Revision as of 00:21, 2 November 2017 by Chrysillispolhaus (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Cleavable peptide sequence from Human tumor necrosis factor ligand superfamily member 13

This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is RKRRAVLT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results of experiments

This peptide is one out of three that were specifically cleaved only by Bitis arietans.

In the graf below you are able to see the cleavage by all three venoms.

Substrate screening data for N17
Figure 1: Substrate with sequence RKRRAVLT (well N17) incubated with the three different venoms.

[edit]
Categories
Parameters
None