Coding

Part:BBa_K2381012

Designed by: Qiqi Xiong   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 23:38, 1 November 2017 by Johnson Huang (Talk | contribs)


3'-OriC gRNA

It’s a gRNA sequence containing 20bp targeting at oriC and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.
The targeting sequence of this part is acquired from xxxx. Its function after J23119 promoter, BBa_K2381020, is proved by OD measurement after cotransformed with plasmid containing dCas9.<img src ="T--HZAU-China--gRNA_function.png" width="200px"> We can observe tight repression on cell growth. Further investigation is conducted by qPCR and flow cytometric.<img src ="T--HZAU-China--gRNA_qPCR.png" width="200px"> Its function after light-induced promoter, BBa_K2381020, is proved by OD measurement. <img src ="T--HZAU-China--light_gRNA.png" width="200px">

References


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part/nominee
Parameters
None