Coding

Part:BBa_K2271000

Designed by: Eric Behle   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 03:47, 2 November 2017 by Anna Sappler (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Pex11

This part contains the coding sequence for the peroxin Pex11p without promoter and terminator to allow altering the strengh of expression.


Usage and Biology

Pex11 is a peroxin located in the inner surface of the peroxisomal membrane and is required for peroxisomal biogenesis [1]. Erdmann and Blobel have shown that the deletion of the PEX11 gene in S. cerevisiae results in cells with fewer, larger peroxisomes, whereas overexpression results in cells with a higher quantity of smaller peroxisomes [2]. This tool provides you with the possibility to gain higher yields at the peroxisomal production of substances.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None