Regulatory

Part:BBa_I751501

Designed by: Kenichiro Iwasaki   Group: iGEM07_Tokyo_Tech   (2007-10-25)
Revision as of 16:40, 26 October 2007 by Iwasaki (Talk | contribs) (→‎Usage and Biology)

plux-cI hybrid promoter

sense TWO INPUTS, activation by AHL and repression by Lambda cI.

Usage and Biology

The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though [http://parts.mit.edu/igem07/index.php/Tokyo/Works/Assay0| Assay 0]. The results are shown in Fig.3. The newly devised hybrid promoter is activated by AHL and repressed by LacI.
In the present project, this hybrid promoter has been tested on the BBa_I751100. (J54140del Pr)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Fig.3: The result of assay0
Only in the presence of AHL and in the absence of lacI, the lux lac hybrid promoter is activated significantly.
[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//regulation/negative
//regulation/multiple
//function/cellsignalling/LuxR
Parameters
negative_regulators1
positive_regulators1