Coding
MazEF

Part:BBa_K2292002

Designed by: FEI CHEN   Group: iGEM17_CCU_Taiwan   (2017-10-26)
Revision as of 22:45, 1 November 2017 by Loorent (Talk | contribs)


Toxin-antitoxin mazEF

MazEF is a suicide system in Escherichia coli which consists of a stable toxin mazF and labile antitoxin mazE. MazF causes cell death by cleaving mRNA at a specific site. On the other hand, mazE plays a role of antitoxin which can antagonize the toxin mazF.

In general, continuous generation of MazE can prevent the situation of cell death by antagonizing mazF. However, once mazE ceased to be produced, it would be degraded by the protease ClpAP. Hence, the remaining toxin mazF will cause the cell death by cleaving mRNA.

<img src="MazEF~truth_23.jpg"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
chassisE. coli
proteinsMazE & MazF