Coding

Part:BBa_K2515003

Designed by: Slavil Peykov   Group: iGEM17_Bulgaria   (2017-10-27)
Revision as of 21:00, 1 November 2017 by Slavil (Talk | contribs)

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Inverted INPUT TetR generator

This part is modified version of Part:BBa_K145201. The J23116_promoter-B0034_RBS-C0040_TetR region from the original part was PCR amplified and cloned inverted to limit the cross-talk with other parts. The new part has no terminators and the LVA-like degradation tag of TetR was removed.

Important!!! This part is inverted!

Here is the page of the original part:
https://parts.igem.org/Part:BBa_K145201

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//direction/reverse
Parameters
None