Coding
Part:BBa_K2194003:Design
Designed by: Catherine Dunaway, Anna Guseva Group: iGEM17_Rice (2017-10-23)
cysPUWA Sulfate Transport System cds
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1274
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2090
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3
Illegal BsaI.rc site found at 3828
Design Notes
[update]
Source
We sourced the nucleotide sequence for cysPUWA from the Escherichia coli MG1655 genome NCBI database (entry NC_000913.3:2539714-2543526). We then sent this sequence to IDT to manufacture as two gBlocks®.