Composite

Part:BBa_K2324009

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-16)
Revision as of 20:13, 1 November 2017 by DanBarber (Talk | contribs)


pRha_FimH_1SynMT

The literature has shown that the terminal pili protein FimH (Le Trong et al 2010) can be modified by inserting heterologous sequences at position 225 and 258 (Pallesen et al 1995, Shembri et al 1999). However these two amino acids are in the pilin binding domain which may present difficulties when attempting to introduce large modifications. Harvard iGEM 2015 also introduced modifications at position 1 of the mature FimH protein.

This part produces a FimH adhesin protein fused with a metallothionein domain from Synechococcus elongatus at the first amino acid position. The coding sequence (BBa_K2324004) is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a FimH+metal binding protein that should initiate pilus biosynthesis when co-transformed with a plasmid containing the fim operon

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1145
    Illegal AgeI site found at 572
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None