Signalling

Part:BBa_K2384008

Designed by: Size Cai   Group: iGEM17_FAFU-CHINA   (2017-10-23)
Revision as of 17:28, 1 November 2017 by Fiona67 (Talk | contribs)


McpB

Chemotaxis is the process by which motile bacteria sense their environment and move towards more favorable surroundings. Environmental ligands are sensed upon binding to transmembrane receptors called methyl-accepting chemotaxis proteins (MCPs).


Usage and Biology

Asparagine chemotaxis in Bacillus subtilis appears to involve two partially redundant adaptation mechanisms: a receptor methylation-independent process that operates at low attractant concentrations and a receptor methylation-dependent process that is required for optimal responses to high concentrations. In order to elucidate these processes, chemotactic responses were assessed for strains expressing methylation-defective mutations in the asparagine receptor, McpB.

Optimization Report

Sequence optimization information:

Expression System: Bacillus megaterium

Gene Length:1986(bp)

Show the relative codon used distribution


Figure 4 :Codon Used Distribution after optimization.



Figure 5:GC Content after optimization.
Figure 3: Digested with EcoRI//.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None