Composite

Part:BBa_K2379005

Designed by: Ramit B   Group: iGEM17_SVCE_CHENNAI   (2017-10-01)
Revision as of 16:29, 1 November 2017 by Azhagammal (Talk | contribs)


RNA thermometer (FourU) with GFP under the regulation of pLAC

The RNA thermometer (BBa_K115002) is under the regulation of pLAC promoter (BBa_R0011). Following the RNA thermometer, the construct contains GFP(BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014). Expression of GFP is regulated by the RNA thermometer and hence will be produced only at 37 degree Celsius.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 765


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Categories
Parameters
None