DNA
Part:BBa_K2243010
Designed by: Li Yulong Group: iGEM17_Peking (2017-10-23)
Revision as of 18:28, 1 November 2017 by Woodsorrel (Talk | contribs)
TP901-1 attB_J23119_TP901-1 attP
The constitutive promoter J23119 is between TP901-1 specific sites and will be inverted by the TP901-1 recombinase, thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase TP901-1 characterization.
We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop.
Figure 2. The standard testing system used to characterize the recombinases.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 105
Illegal NheI site found at 128 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 188
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |