Composite

Part:BBa_K2348012

Designed by: Andreas Berner   Group: iGEM17_NAWI_Graz   (2017-10-19)
Revision as of 16:22, 1 November 2017 by Andi332 (Talk | contribs)


alx promoter and riboswitch + mNeonGreen
This part actually is a composite part containing the alx promoter and riboswitch BBa_K2348000 and mNeonGreen BBa_K1761003. But in addition, between riboswitch and mNeonGreen a TEV-site and a f-degron are inserted. Also, mNeonGreen ins 6xHis-taged and the parts ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and f-degron allow a fast and controllable degradation of mNeonGreen and in combination with the fast maturation time of mNeonGreen allow a fast colour change when used together with our other pH-sensitive part BBa_K2348011


Sequence and Features
799px-Alx_part_Graz.jpeg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 312


[edit]
Categories
Parameters
None