Coding

Part:BBa_K2398559:Design

Designed by: Thore Buergel, Lukas Adam, Catharina Gandor, Marita Klein, Jan Mathony, Pauline Pfuderer, Lukas Platz, Moritz Przybilla, Max Schwendemann, Julius Upmeier zu Belzen   Group: iGEM17_Heidelberg   (2017-10-27)
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Beta-Glucuronidase_T509A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 506
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created from the wildtype E.coli beta-glucuronidase. One or more amino acid substitutions were introduced. It was used for protein expression under a lac inducible T7 promoter.


Source

This part was modified from the beta-glucuronidase coding sequence of E. coli, obtained from AddGene (#61156).

References