Part:BBa_K190031

fMT with low constitutive promoter

The metallothionein fMT under control of a low constitutive promotor BBa_J23109

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 81
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 170
  
• 1000
  COMPATIBLE WITH RFC[1000]

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Contribution

Group: NCTU_Formosa 2017

Author: Yi-Ting Lin, Huei-Yu Yeh, Sheng-Ping Chu

Summary:
The metallothionein (BBa_K190031) is a fMt(BBa_K190019) under control of a low constitutive promotor (BBa_J23109). We failed several times in replicating the ligation of these two parts. After sequencing BBa_K190031, BBa_K190019, and BBa_J23109, we found the constitutive promoter BBa_J23109 has two Spel restriction sites in the prefix.(Figure 2.) Thus, we decided to modify the biobrick by ligating fMt(BBa_K190019) with another constitutive promoter (BBa_J23119). Figure 3 shows the electrophoresis of BBa_K190019 when its plasmid was cut by XbaI and PstI. And Figure 4 shows the electrophoresis of BBa_J23119 and BBa_J23109 when their plasmids were cut by SpeI and PstI.