Device

Part:BBa_K2205018

Designed by: Lais Takiguchi   Group: iGEM17_Newcastle   (2017-09-21)
Revision as of 10:20, 1 November 2017 by Laistakiguchi (Talk | contribs)


Sensynova Framework (Connector 2B + spisPink (Complete) - rCell)

Description here

This part was made to testing the modularity of the system by replacing the sfGFP reporter module of the Sensynova platform design with the chromoprotein variants spisPink (BBa_K1033925).

The spisPink protein is a pink chromoprotein extracted from the coral Stylophora pistillata. It was first extracted and characterized by Alieva et al. under the name spisCP (GenBank: ABB17971.1) and codon optimized for E. coli by Genscript. The protein has an absorption maximum at 560 nm giving it a pink colour visible to the naked eye. The strong colour is readily observed in both LB or on agar plates after less than 24 hours of incubation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776


[edit]
Categories
Parameters
None