Coding

Part:BBa_K2317001

Designed by: Shan Wang   Group: iGEM17_Jilin_China   (2017-10-20)
Revision as of 10:46, 1 November 2017 by JinLiu (Talk | contribs)


CbtA

CbeA-CbtA is one of the Escherichia coli TA systems, and the Toxin, CbtA, has been reported to inhibit the polymerization of bacterial cytoskeletal proteins, MreB and FtsZ.(Fugure 1)

Figure 1. The mechanism of CbeA and CbtA(type IV TA system).


Characterization:

The population and growth condition can be reflected by Abs600, so through the curve of Abs600-time, the effect of CbeA and CbtA towards bacteria can be displayed. Abs600 values were measured from 0 to 14 hours in two groups and the toxication curves were drawn as shown in Figure 2. The growth rate of vector group bacteria after adding L-Arabinose sped up for a short time and then slowed down. At last, the trend was consistent with that without L-Arabinose. However, TA group, with the addition of L-Arabinose, showed the same OD and Abs600 values from the 4th hour for a while. After that, Abs600 values displayed a little increase.

File:"T-Jilin China--fg2.png
Figure 2. (A) Different concentrations of L-Arabinose were added into vector group and the growth curve was drawn to made sure the effect of L-Arabinose towards bacteria. According to the results, the addition of L-Arabinose would first slightly enhance and then suppress the growth. However, this effect would disappear after a while. (B) Different concentrations of L-Arabinose were added into TA group and the growth curve was drawn to made sure the effect of CbtA(induced by L-Arabinose) towards bacteria. According to the results, the addition of LArabinose the growth would be stagnated at first and then recover. Fig.

Besides, after culturing for 14 hrs, difference in turbidities of bacteria in different groups was visible Figure 3.

Figure 3. (A) and (B) The two figures were taken to compare the turbidities of TA group after 14 hours od culturing, and the difference could be told by eyes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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